Cells in logarithmic growth pHase were placed in refrigerator for gradient cooling and then were preserved in liquid Nitrogen (-196 ℃) for 2~ 6 months, the viability of cells was determined by trypan-blue dye, cell morpHology were also observed. 选取处于对数生长期的培养细胞梯度降温后置入液氮(-196℃)中保存4~6个月,进行细胞复苏培养,以2%台盼蓝确定细胞活力,并观察复苏的成纤维细胞形态学状况。
The physiological and biochemical change of host during initial interaction phase between LA-toxin and suspension cell were studied by using suspension cell within logarithmic growth phase. 以处于对数生长初期的悬浮培养细胞为试验材料,研究了在悬浮细胞与毒素亲和性与非亲和性互作早期阶段寄主细胞生理生化的变化。
In all experiments, cells were used in logarithmic growth phase. 所有实验均采用对数生长期细胞。
Beijing-7 strains of varicella-zoster virus were inoculated in human embryo lung diploid cells which were in logarithmic growth phase. When CPE appeared in the cells infected with VZV, pre-existent maintenance media was exchanged by MEM which contained no bovine serum. 选择处于对数生长期的人胎肺二倍体细胞(2BS),接种北京-7株水痘-带状疱疹病毒.待受染病毒的细胞出现CPE时,将维持液换为不含牛血清的MEM。
It was found that there was good linear relationship between fermenting rate in logarithmic growth phase and glucose concentration and slope and intercept of fermentation rate equation was linear with the initial glucose concentration. 发现细胞生长期发酵速率与基质浓度间存在良好的线性关系,发酵速率方程的斜率和截距与基质初始浓度也存在良好的线性关系;
The first, algae promote the formation and diposition of phosphate by change of the pH; the balance system of ions; and the oxidation-reduction potential ( Eh) in environment during the logarithmic growth phase. 其一是改变环境的pH值、离子平衡系统和氧化还原电位促进磷酸盐形成并沉淀;
The experiment was performed during the logarithmic growth phase of cells. 细胞处于对数生长期时进行实验。
Rat myocardial cells ( H9C2) by in vitro culture, using the logarithmic growth phase cells as experimental treatment. 大鼠心肌细胞(H9C2)经体外培养扩增,使用对数生长期细胞做实验处理。
Cells in logarithmic growth phase were used in experiments. 3. 收集对数生长期细胞用于实验。
But under the light condition, in the logarithmic growth phase the extracellular protease activity increased rapidly with the multiplication of bacteria. 而在厌氧光照培养条件下,在对数生长期阶段胞外蛋白酶活力随细菌快速繁殖而迅速升高。
Cells in the logarithmic growth phase were randomly divided into normal control group, injury group and Equisetum extract group. 取对数生长期细胞,随机分成对照组、氧化损伤组、木贼提取物干预组。
Logarithmic growth phase of C6 glioma cells were seeded in serum-free medium in order to acquire BTSCs. 方法1.取对数生长期的C6胶质瘤细胞接种于无血清培养基,获得肿瘤干细胞。